Evaluation of a 200-kDa amastigote-specific antigen of L. donovani by enzyme-linked immunosorbent assay (ELISA) for the diagnosis of visceral leishmaniasis

✍ Scribed by Pankaj Kaul; N. Malla; Sukhbir Kaur; R.C. Mahajan; N.K. Ganguly

• Publisher: Elsevier Science
• Year: 2000
• Tongue: English
• Weight: 507 KB
• Volume: 94
• Category: Article
• ISSN: 0035-9203
• DOI: 10.1016/s0035-9203(00)90264-5

✦ Synopsis

A purified 200~kDa antigenic fraction from Leishmania donovani axenic amastigotes was evaluated by ELISA for the detection of antibody response in visceral leishmaniasis (VL) patients, post kala-azar dermal leishmaniasis (PKDL) patients and controls, for the diagnosis of visceral leishmaniasis. A positive antibody response to the 200~kDa amastigote fraction and to Leishmaniu amastigote soluble antigen (LASA) was found in 29 (96.6%) and 30 ((loo"/) o confirmed VL patients, respectively, by the use of ELISA. However, only 1 (10%) out of 10 PKDLpatients had detectable antibody response to 200~kDa fraction while all the 10 (100%) PKDL patients exhibited an immune response to LASA. Therefore, use of the 200-kDa antigenic fraction for the detection of antibody response in an ELISA follow-up (post treatment) of VL patients may have prognostic significance, and it may also be useful for differentiating active VL and PKDL.