Recent studies have provided theoretical and empirical evidence about the importance of hatchling production for sea turtle population dynamics. Therefore, understanding the effect of nesting habitat loss as a factor leading to hatchling reduction is essential in order to establish conservation plans for the recovery of sea turtle populations. In the present study, we developed a method to quantify habitat loss and link it with hatchling production. We used data for loggerhead sea turtles (Caretta caretta) collected at Sekania nesting beach, western Greece, to describe biological and behavioral attributes of nesting individuals. Spatial characteristics of the nesting site were analyzed and alternative scenarios of habitat loss were examined. We then used circle-packing technique to evaluate the impact of an increasingly reduced available nesting area on the spatial distribution of nests. An increased number of nests within the study site resulted in density-dependent processes regulating hatchling production. Under the different scenarios, we evaluated the risk of the laying nests exceeding the estimated carrying capacity of the nesting beach. Our results clearly demonstrated the need to apply direct and efficient conservation measures at Sekania nesting site to minimize further habitat loss from human-related processes and a rising sea level. The approach developed evaluates the effect of habitat loss upon nesting by linking it with quantifiable processes (density dependence), providing a conservation tool to guide planning decisions towards the conservation of the sea turtle population.