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Eukaryotic gene cloning and expression in yeast

โœ Scribed by Nasmyth, Kim

Book ID
109710481
Publisher
Nature Publishing Group
Year
1978
Tongue
English
Weight
390 KB
Volume
274
Category
Article
ISSN
0028-0836

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We developed a host-vector system for transformation and gene cloning experiments using the methylotrophic yeast Hansenula p o l y w p h a . Regeneration of protoplasts in a medium containing polyethylene glycol before plating made transformation more efficient and reproducible (2 to 3 -lo4 pg DNA).