Eukaryotic cloning vectors based on bovine papilloma viruses

✍ Scribed by Daniel DiMaio

• Book ID: 102758237
• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 505 KB
• Volume: 1
• Category: Article
• ISSN: 0265-9247
• DOI: 10.1002/bies.950010110

✦ Synopsis

The cloning of eukaryotic genes by standard recombinant D N A techniques permits their structural characterization. However, analysis of the expression properties of these genes often requires their introduction into and replication within eukaryotic cells in culture. Certain viral vectors based on the papilloma viruses may prove to be especially important in such investigations. These 'shuttle vectors', capable of replication in both bacterial and eukaryotic cells, have already provided several jindings of interest about the relationship between eukaryotic gene structure and f i c t i o n . Further analysis of these vectors and their properties promises to enhance their use as investigative tools.