Euglena gracilischloroplast DNA: analysis of a 1.6 kb intron of thepsbC gene containing an open reading frame of 458 codons

✍ Scribed by Paul-Etienne Montandon; Alain Vasserot; Erhard Stutz

• Publisher: Springer-Verlag
• Year: 1986
• Tongue: English
• Weight: 532 KB
• Volume: 11
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00389423

✦ Synopsis

We retrieved a 1.6 kbp intron separating two exons of the psb C gene which codes for the 44 kDa reaction center protein of photosystem II. This intron is 3 to 4 times the size of all previously sequenced Euglena gracilis chloroplast introns. It contains an open reading frame of 458 codons potentially coding for a basic protein of 54 kDa of yet unknown function. The intron boundaries follow consensus sequences established for chloroplast introns related to class II and nuclear pre-mRNA introns. Its 3'-terminal segment has structural features similar to class II mitochondrial introns with an invariant base A as possible branch point for lariat formation.