Etude des propriétés électro-optiques de i' ADN par biréfringence électrique à faible champ, 1. Théorie, appareillage et exploitation des résultats

✍ Scribed by Marion, Christian ;Roux, Bernard ;Bernengo, Jean-Claude

• Publisher: Wiley (John Wiley & Sons)
• Year: 1984
• Weight: 813 KB
• Volume: 185
• Category: Article
• ISSN: 0025-116X
• DOI: 10.1002/macp.1984.021850814

✦ Synopsis

Electric birefringence (or Kerr effect) measurements can give, in principle, important informations on the optical, electrical and hydrodynamical properties of macromolecules. The theoretical basis of the Kerr effect is reviewed here so as to be applied to the determination of some molecular parameters of DNA such as optical and electrical polarizability anisotropies, permanent dipole moment, and rotatory diffusion constant. It is necessary to conduct the measurements at very low electric fields. The original device used and the methods for the analysis of the experimental data are described.