Ethoxyresorufin-O-deethylase and Porphyrin Analysis in Chicken Embryo Hepatocyte Cultures with a Fluorescence Multiwell Plate Reader

✍ Scribed by S.W. Kennedy; A. Lorenzen; C.A. James; B.T. Collins

• Book ID: 102966337
• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 1000 KB
• Volume: 211
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1993.1239

✦ Synopsis

Rapid and sensitive methods for measuring ethoxyresorufin-O-deethylase (EROD) induction and total intracellular porphyrin accumulation in cultured chicken embryo hepatocytes exposed to polychlorinated biphenyls (PCBs) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are described. Cells were cultured in 48 -well cell culture plates, and EROD assays were carried out directly within the wells. EROD activity and porphyrin concentrations were determined with a computer-operated fluorescence multiwell plate reader. Porphyrin and EROD assays could be carried out on the same plates or on separate plates. The assays allow one to obtain dose-response curves at rates that are approximately 100 times faster than conventional methods.

(c) 1993 Academic Press, Inc.