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Ethical storm brews over embryonic stem cell lines


Book ID
122709818
Publisher
Reed Business
Year
2008
Tongue
English
Weight
92 KB
Volume
199
Category
Article
ISSN
0262-4079

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A total of 14 microsurgically produced zona pellucida-free bovine demi-blastocysts were cultured for 3 days in tissue culture medium (TCM) 199 supplemented with 10% heat-inactivated newborn calf serum (NBCS). Developing embryos were continuously cultured in TCM 199 plus 10% NBCS on a feeder-layer of