Ether and ester glucuronic acid conjugates of lacidipine metabolites: Mass spectrometric differentiation using thermospray and particle beam techniques
✍ Scribed by P. Rossato; M. Scandola; F. Pugnaghi; P. Grossi
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 391 KB
- Volume
- 27
- Category
- Article
- ISSN
- 1076-5174
No coin nor oath required. For personal study only.
✦ Synopsis
Glucuronic acid conjugates of Lacidipine metabolites were analysed by high-performance liquid chromatography/mass spectrometry with different ionization techniques, viz. thermospray and cbemical ionization with a particle beam interface. Analyses were performed on bile samples collected from dogs after oral administration of I4C-labelled Lacidipine. Phase I1 metabolites, originating from the conjugation with glucuronic acid of phase I metabolites containing free hydroxy or carboxylic functions, were isolated. Two types of glucuronides, with an ether or an ester bond, were characterized. They showed different mass spectrometric behaviour owing to a specific interaction with the acetate in the mobile phase.