Ethanolic extraction, purification, and partial characterization of a fluorescent toxin from the coral reef crab, Lophozozymus pictor
✍ Scribed by C. O. Lau; C. H. Tan; H. E. Khoo; Q. T. Li; R. Yuen
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 359 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1056-9014
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✦ Synopsis
A purification procedure for Lophozozymus pictortoxin (IPTX) following ethanolic extraction of whole crab homogenate is described. The ethanol-extracted toxin (LPTX-E) had higher yield and specific activity than the hot aqueous-extracted one (LPTX-H). It was found that LPTX-E was fluorescent and cochromatographed with LPTX-H on two-dimensional thin-layer chromatography. Although LPTX-E, LPTX-H, and palytoxin (P. caribaeorum, PTX) had similar migration and retention times when analysed on high performance capillary electrophoresis and gel permeation-high performance liquid chromatography respectively, LPTX-E and LPTX-H were both fluorescent in contrast to PTX. In addition, LPTX-E had a different retention time compared with PTX when chromatographed on reversed phase high performance liquid chromatography in the solvent system 80% acetonitrile and 0.02 M Tris-HCI, pH 7.2, at a 4:l ratio, respectively, indicating some differences in their chemical structures. o 199s wiley-Liss, Inc.