Ethanol tolerance and activity of plasma membrane ATPase in Kluyveromyces marxianus and Saccharomyces cerevisiae
✍ Scribed by M.Fernanda Rosa; Isabel Sá-Correia
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 455 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0141-0229
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📜 SIMILAR VOLUMES
Ethanol depressed the maximum temperature for growth (Tmax) and enhanced thermal death in the yeasts Saccharomyces cerevi~iael-~ and Kluyveromyces f r a g i l i ~. ~ Information obtained from brewers on difficulties with yeast performance in the brewing at low temperatures of lager from high gravity
Partially (6-fold) purified plasma membrane ATPase from an ethanol-sensitive yeast, Kloeckera apiculata, had an optimum pH of 6.0, an optimum temperature of 35°C, a K m of 3.6 mM ATP and a V max of 11 μmol Pi/min.mg protein. SDS-PAGE of the semi-purified plasma membrane showed a major band of 106 kD