Ethanol production from fructose in continuous culture by free and flocculent cells ofZymomonas mobilis
β Scribed by I. Toran-Diaz; V. K. Jain; J. C. Baratti
- Publisher
- Springer Netherlands
- Year
- 1984
- Tongue
- English
- Weight
- 414 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0141-5492
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β¦ Synopsis
SuMMARk'
Zymomonas mobilis st,rei.n ZM4 was used for ethanol production from fructose (100 g/l) in continuous culture with a mineral (cant eining Ca pantothenate) or a rich (containing yeast extract) mediium. With both media high conversion yields we,re observed but the ethanol productivity was limited by the low biomass content of the fe-lilentor.
A new flocculent strain of Zmobilis (ZM4F) was cultivated in a CSTR with an internal settler and showed a maximal productivity of 93 g/l.h (fructose conversion of 8Cr%). When the fructose conversion was 96% en ethanol productivity of 85.6 g/l.h with an ethanol yield of 0.49 g/g (96% of theoretical) was observed.
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For the continuous, enzymatic synthesis of sorbitol and gluconic acid by cell-free glucose-fructose oxidoreductase (GFOR) from Zymomonas mobilis, the principal determinants of productivity have been identified. Most important, the rapid inactivation of the soluble enzyme during substrate conversion