Ethanol production from corn cob pretreated by the ammonia steeping process using genetically engineered yeast
✍ Scribed by N. J. Cao; M. S. Krishnan; J. X. Du; C. S. Gong; N. W. Y. Ho; Z. D. Chen; G. T. Tsao
- Book ID
- 104633683
- Publisher
- Springer Netherlands
- Year
- 1996
- Tongue
- English
- Weight
- 336 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0141-5492
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✦ Synopsis
A new and effective pretreatment process for biomass conversion involves the steeping of biomass in 2.9 M Nt-hOH. This resulted in the removing about 80-90% of the lignin along with almost all the acetate from cellulosic residues. Based on dry cellulose from corn cob, a high glucose yield of 92% was obtained after enzymatic saccharification of cellulose fraction. By using a genetically engineered, xylosefermenting Saccharomyces 1400(pLNH33) in the batch fermentation of a glucose-xylose mixture from corn cob, an ethanol concentration of 47 g/L was obtained within 36 h with 84% yield. In addition, an ethanol concentration of 45 g/L was obtained within 48 h with 86% yield using simultaneous saccharification-fermentation process.