The effects of the natural polyamines, putrescine, spermidine and spermine on single calcium-activated potassium channels from clonal rat pituitary tumor cells (GH3) were studied. Applied to inside-out patches, polyamines were found to reduce the current amplitude and open probability of the channel
Ethanol Activates Maxi Ca2+-activated K+Channels of Clonal Pituitary (GH3) Cells
β Scribed by M. Jakab; T.M. Weiger; A. Hermann
- Publisher
- Springer
- Year
- 1997
- Tongue
- English
- Weight
- 609 KB
- Volume
- 157
- Category
- Article
- ISSN
- 0022-2631
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π SIMILAR VOLUMES
Single Ca2(+)-activated K+ channels were studied in membrane patches from the GH3 anterior pituitary cell line. We have previously demonstrated the coexistence of large-conductance and small-conductance (280 pS and 11 pS in symmetrical 150 mM K+, respectively) Ca2(+)-activated K+ channels in this ce
A large-conductance, Ca 2/ -activated K / channel was identified and characterized in embryonic chick hepatocytes using the patch-electrode voltage-clamp technique. The channel conductance was 213 pS in excised patches bathed in symmetrical 145 mM KCl and 1 mM Ca 2/ . Current-voltage relationships w