Ethane exhalation and vitamin E/ubiquinol status as markers of lipid peroxidation in ferrocene iron—loaded rats
✍ Scribed by Bernd Dresow; Claudia Albert; Inge Zimmermann; Peter Nielsen
- Publisher
- John Wiley and Sons
- Year
- 1995
- Tongue
- English
- Weight
- 779 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0270-9139
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✦ Synopsis
Organ damage caused by iron overload has been mostly attributed to iron-induced peroxidation of membrane lipids. Using the ferrocene iron-loaded rat model, we studied ethane exhalation as a direct marker of in vivo lipid peroxidation, as well as concentrations of atocopherol and ubiquinol 9/10 in liver and plasma as indirect markers of this process. The feeding of a diet enriched with 0.5% TMH-ferrocene up to 31 weeks resulted in a large increase in liver iron concentration to about 25 mg/g wet weight (w wt). At lower, predominantly hepatocellular liver siderosis, the breath ethane exhalation was dependent on dietary vitamin E CVitE) supplements (onset of ethane exhalation at liver-Fe >2 mg/g w wt on vitE-restricted diet; >5 mg Fe per gram on VitE-replete diet). At severe liver siderosis, breath ethane exhalation reached a maximum of approximately 8 nmol/kg/hr independent of VitE supplementation. Plasma as well as hepatic a-tocopherol decreased with progressive iron loading. In addition, a significant depletion in hepatic ubiquinol9 and 10 was noted.