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Estrogen receptor of calf uterus: An easy and fast purification procedure

✍ Scribed by G.A. Puca; N. Medici; A.M. Molinari; B. Moncharmont; E. Nola; V. Sica


Book ID
116002075
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
933 KB
Volume
12
Category
Article
ISSN
0022-4731

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Glutathione reductase has been purified to at least 98% homogeneity from calf liver. An essential part in the procedure involves affinity chromatography on 2',5'-ADP-Sepharose 4B to which the enzyme remains bound in the presence of 0.4 M phosphate. This step separates glutathione reductase from the