Estrogen receptor-mediated cytotoxicity using iodine-125

✍ Scribed by W. D. Bloomer; W. H. McLaughlin; R. A. Milius; R. R. Weichselbaum; S. J. Adelstein

• Book ID: 102301889
• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: English
• Weight: 329 KB
• Volume: 21
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.240210106

✦ Synopsis

Auger effects from 1251 decay are singularly damaging if localized in DNA as the thymidine analogue 1251-iododeoxyuridine ( '251UdR). Recent experience with steroid sex hormones extends these observations by demonstrating cytotoxicity in sites other than the DNA backbone. We have compared the cytotoxicity in human MCF-7 breast cancer cells of '251UdR, '251-iodotamoxifen, a nonsteroidal antiestrogen that is translocated from the cytoplasm to the nucleus of receptor containing cells, and 1251-iodoantipyrine, a biological indicator of the body water space. Cytotoxicity is critically dependent upon subcellular localization.