Estrogen-mediated induction of a vitellogenin-specific nonhistone chromatin protein in the male chicken liver
✍ Scribed by Nakayama, Tatsuo ;Irikura, Maki ;Setoguchi, Yoshiko ;Nakayama, Masami ;Mochizuki, Mitsuyoshi ;Ogata, Kikuo
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 692 KB
- Volume
- 201
- Category
- Article
- ISSN
- 0026-8925
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✦ Synopsis
Non-histone chromatin proteins prepared from the livers of estrogen-treated and nontreated male chickens were compared by reverse-phase high performance liquid chromatography (RP-HPLC), followed by sodium dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The results revealed that the hormone-treated male liver chromatin contained a specific protein corresponding to the vitellogenin-specific protein previously purified from the liver of egg-laying hens (Nakayama 1985). The chicken protein, purified further by gel-filtration high performance liquid chromatography (GF-HPLC), showed specific binding activity to DNA fragments carrying a part of the vitellogenin gene. On the basis of similarities in the elution patterns from GF-HPLC and RP-HPLC as well as in the mobility on SDS-PAGE, we concluded that this hormone-induced protein in the male chicken liver was identical to the vitellogenin-specific protein identified in the hen liver, and assumed it to be a specific regulatory protein for the vitellogenin gene expression. The amino acid composition of this chicken protein has been determined.