Estradiol-dependent trans-acting factor binds preferentially to a dyad-symmetry structure within the third intron of the avian vitellogenin gene
✍ Scribed by Jean-Pierre Jost; Hanspeter Saluz; Josef Jiricny; Bruno Moncharmont
- Book ID
- 102880207
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 950 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
The secondary activation of the avian vitellogenin II gene in isolated liver nuclei by cytoplasmatic liver extracts of estradiol-treated chicks is accompanied by the binding of a protein from the extract to the structural part of the cloned gene. Both the DNA-binding and gene-stimulatory activities, which cochromatograph on heparin-Sepharose, are apparently present only in the cytoplasmatic liver extracts of estradiol-treated roosters and in the oviduct extracts of egg-laying hens. DNA-binding competition assays combined with exonuclease I11 footprinting showed that the factor binds to the imperfect dyad-symmetry structure "GTCTTGTTCCAAAC3' within the third intron of the gene. The factor is sequence specific and binds equally well to both single-and double-stranded DNA with an estimated dissociation constant of 3.5 x M.