Evaluation of estradiol binding in human
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S. Ganesan; Dr. N. Bashirelahi; J. D. Young
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Article
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1988
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John Wiley and Sons
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English
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We have systematically evaluated the effect of trislphosphate buffers, ethylenediamine tetraacetate (EDTA), dithiothreitol (DTT), molybdate, and phenylmethylsulfonyl fluoride (PMSF) on the binding of 170estradiol to human BPH cytosol. Competition binding assays demonstrated that the estrogen recepto