Estimation of types I and III collagens in whole tissue by quantitation of CNBr peptides on SDS-polyacrilamide gels

Quantitation of type I, III, and V colla

Quantitation of type I, III, and V collagens in human tissue samples by high-performance liquid chromatography of selected cyanogen bromide peptides

✍ Edward J. Miller; Donald K. Furuto; Annie J. Narkates 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 779 KB

A method to determine the proportions of the major fiber-forming collagens (types I, III, and V) in noncartilaginous human tissues is presented. The procedure relies on direct solubilization of tissue collagen as cyanogen bromide peptides. The peptides are subjected to cation exchange chromatography