Estimation of the proportion of inbred seed in Brussels sprouts hybrid seed by acid phosphate isoenzyme analysis

✍ Scribed by B. Te Nijenhuis

• Publisher: Springer
• Year: 1971
• Tongue: English
• Weight: 480 KB
• Volume: 20
• Category: Article
• ISSN: 0014-2336
• DOI: 10.1007/bf00034204

✦ Synopsis

The acid phosphatase isoenzyme patterns of extracts of seeds from Brassica oleracea subspecies and Brussels sprouts cultivars have been analysed . In view of the inhomogeneity of most seed material, the extracts must be prepared from single seeds if the patterns are to be used for taxonomic classification .

The acid phosphatase isoenzymes located in the 15-20 mm region of the electropherograms proved to be controlled by one locus, which consists of 4 alleles acting without dominance. As a result, the acid phosphatase patterns of seeds of pure inbred lines are characterized by a single band in this region . Crossing of inbred lines with a different allele constitution gives hybrid seed with the related double-band pattern. No polypeptide hybridization occurs .

By analysis of the acid phosphatase patterns of sufficient individual seeds in a seed sample from a new Brussels sprouts hybrid, it was possible to determine the proportion of inbred seed in the sample . The results were in good agreement with those obtained from field observations .