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Estimation of the position and effect of a lethal factor locus on a molecular marker linkage map

✍ Scribed by R. Cheng; A. Saito; Y. Takano; Y. Ukai

Publisher
Springer
Year
1996
Tongue
English
Weight
916 KB
Volume
93
Category
Article
ISSN
0040-5752

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✦ Synopsis

In the mapping of DNA markers the distortion of segregation of marker genotypes is often observed, which may be caused by a lethal factor acting in filial generations derived from distant crosses. A method is presented for estimating the recombination values between a lethal factor locus and neighboring molecular markers, and the relative viability or fertilization ability of gametes or zygotes affected by the lethal factor in an F 2 population using the maximum likelihood method and the expectation conditional maximization (ECM) algorithm. Three selection models of gamete or zygote were considered, and the most likely one was determined by goodness of fit of the observed frequency of the phenotypes to the expected ones under the models. The method was applied to segregation data of molecular markers of an F 2 population consisting of 144 individuals derived from a cross between an Indica and a Japonica rice variety. The presence of a lethal factor locus (L) located on chromosome III that caused partial gametic selection in both the male and female sides was suggested. The locus L was tightly linked to RFLP marker number 23 of the RFLP linkage map of Saito et al. (1991a), and the fertilization chance of a male or female gamete possessing the lethal factor was, on average, 4l .5% of that of the normal gamete. Key words Mapping. Segregation distortion 9 Molecular marker 9 Recombination value 9 Maximum likelihood method Communicated by G. Wenzel R. Cheng ([]).

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