Estimation of the mechanical property of meniscus using ultrasound: Examinations of native meniscus and effects of enzymatic digestion

✍ Scribed by Ko Yasura; Yasuyuki Mizuno; Yasuaki Nakagawa; Koji Mori; Makoto Takenaka; Tetsuo Ohashi; Keisuke Yamada; Masahiko Kobayashi; Keiji Ando; Hiroshi Kuroki; Takashi Suzuki; Ken Ikeuchi; Sadami Tsutsumi; Takashi Nakamura

• Publisher: Elsevier Science
• Year: 2007
• Tongue: English
• Weight: 304 KB
• Volume: 25
• Category: Article
• ISSN: 0736-0266
• DOI: 10.1002/jor.20256

✦ Synopsis

Abstract

We previously developed a novel ultrasound assessment system featuring wavelet transform to evaluate the material properties of articular cartilage. We aimed in this study to demonstrate the feasibility of quantitative evaluation of meniscus using ultrasound and to elucidate the relationships between its acoustic, mechanical, and biochemical properties. Meniscal disc specimens from mature pigs were assessed by ultrasound and compression testing, and their correlation was analyzed. A positive correlation was found between the ultrasound signal intensity and apparent Young's modulus (r = 0.61). Subsequently, the porcine meniscal discs were treated with various enzymes and then characterized by ultrasound, by compression tests, by biochemical analyses, and by histology and immunohistochemistry. The signal intensity was decreased not by hyaluronidase but by collagenase treatment. Hyaluronidase‐treated menisci showed a discrepancy between acoustic and mechanical properties, suggesting that the ultrasound reflection could not detect a reduction in proteoglycan content. Also, ultrasound signal intensity could only reflect superficial layers of the material. Several limitations exist at present, and further studies and improvements of the device are required. However, given the noninvasive nature and the requirement of only small equipment, this ultrasound assessment system will be an instrumental diagnostic tool for meniscal function in both research and clinical fields. © 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25:884–893, 2007