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Estimation of the homoplasmy degree for transplastomic tobacco using quantitative real-time PCR

✍ Scribed by Huifeng Shen; Bingjun Qian; Litao Yang; Wanqi Liang; Weiwei Chen; Zhenhua Liu; Dabing Zhang

Publisher
Springer
Year
2010
Tongue
English
Weight
324 KB
Volume
231
Category
Article
ISSN
0044-3026

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## Abstract Real‐time quantitative polymerase chain reaction (PCR) on the LightCycler instrument (LC‐PCR) was developed to measure the Epstein‐Barr virus (EBV) load in clinical samples. LC‐PCR detected two copies of the EBV genome per 500 ng of DNA. Its specificity was confirmed by assays in EBV‐ne