Estimation of picomole quantities of piperidine in tissues

✍ Scribed by N. Seiler; H. H. Schneider

• Book ID: 102772342
• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: English
• Weight: 559 KB
• Volume: 1
• Category: Article
• ISSN: 1076-5174
• DOI: 10.1002/bms.1200010604

✦ Synopsis

Piperidine 'Is a putative neurotransmitter. Its sensitive determination is one of the prerequisites for the evaluation of its assumed physiological role. For its derivatization 5-di-n-butylaminonaphthalene-1-sulphonyl chloride, a new reagent which improves methods applying 5-dimethylaminonaphthalene-I-sulphonyl chloride, is used. Reaction of the perchloric acid tissue extract with this reagent, thin-layer chromatographic separation of the fluorescent derivatives and mass spectrometric evaluation of the di-n-butylaminonaphthalenesulphonyl piperidine spot by a version of the integrated ion current technique allows the determination of piperidine in tissues in picomole quantities. Standard deviation of the determinations of 2 to 16 pmol of piperidine added to 50 mg of brain tissue was 8 %. The method was applied to the analysis of different tissues. Brain concentrations of piperidine in different species were found to be lower by a factor up to 100 in comparison with published data.