Estimation of Phosphorus and Sulphur Contents in Nuclear Constituents by Means of Image-EELS Analysis Using Ribosomes as Internal Standards
✍ Scribed by S. Abolhassani-Dadras; J. Mayer; S. Fakan
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 441 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0968-4328
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✦ Synopsis
A quantitative study by means of electron energy-loss spectroscopic imaging (image-EELS) is performed on a biological specimen, using ribosomes as internal standard. Phosphorus and sulphur are studied; it is shown in the case of phosphorus analysis that results obtained are in agreement with a previous study using a different energy filtering microscope.
In most biological specimens, atoms of sulphur may be present in the vicinity of phosphorus atoms; in such cases the signal obtained from sulphur will overlap with the continuum coming from the phosphorus signal, thus making the element-specific maps of sulphur superimposed on a background signal coming from phosphorus. In this work a method is suggested of separating the phosphorus contribution to the sulphur signal, using a reference spectrum from gallium phosphide.