Estimation of parameters in double uptake systems

✍ Scribed by H.T. Hutchison; B. Haber

• Publisher: Elsevier Science
• Year: 1975
• Tongue: English
• Weight: 426 KB
• Volume: 68
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(75)90657-0

✦ Synopsis

A method is presented for estimating the kinetic parameters describing the cellular uptake of a single molecular species mediated by two independent transport processes. Two cases are considered: first, uptake by two carrier-mediated transport systems, and second, uptake by a single carrier-mediated system and diffusion. Unweighted and weighted least-squares estimations are described: the unweighted estimation is noniterative and provides excellent first approximations for the iterative weighted estimation procedure. K, + s K,+s'

(1) where s is the substrate concentration, V, and V, are the maximim velocities of uptake, and K, and K2 are the substrate concentrations for 617