Estimation of P-to-O ratio in Bacillus subtilis and its influence on maximum riboflavin yield
✍ Scribed by Uwe Sauer; James E. Bailey
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 95 KB
- Volume
- 64
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
Simultaneous growth and riboflavin overproduction were investigated using a previously developed stoichiometric model of Bacillus subtilis metabolism. A fit of model predictions to experimental data was used to obtain estimates of fundamental energetic parameters of B. subtilis. Although multiple solutions describe the experimental data, evidence for a P-to-O ratio of about 1 1 ⁄3 mole of ATP produced per atom of oxygen consumed in oxidative phosphorylation was provided by genomic analysis of electron transport components, because no homologue of the proton-translocating NADH dehydrogenase I was found in the B. subtilis genome database. These results allow us to devise a rational metabolic engineering strategy to improve riboflavin production. The potential influence of increased energy coupling in oxidative phosphorylation on riboflavin yield is discussed. Higher coupling is most significant under carbon-limiting conditions in slow-growing cells, that is, in fed-batch processes of industrial interest.