Estimation of intramitochondrial pCa and pH by fura-2 and 2,7 biscarboxyethyl-5(6)-carboxyfluorescein (BCECF) fluorescence
β Scribed by Michael H. Davis; Ruth A. Altschuld; Dennis W. Jung; Gerald P. Brierley
- Book ID
- 115761026
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 390 KB
- Volume
- 149
- Category
- Article
- ISSN
- 0006-291X
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π SIMILAR VOLUMES
2 0 ,7 0 -Bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) is a fluorescent probe used to examine multidrug resistance-associated protein (MRP) transporter activity in cells. BCECF is introduced into the cell as the nonfluorescent membrane permeable acetoxymethyl ester, BCECF-AM, where it is hydr
The dyes carboxy-SNARF-1 and BCECF are fluorescent probes of intracellular pH that exhibit changes in spectral shape upon proton binding which allow one to use measurements of fluorescence at two or more wavelengths in order to measure pH without artifacts associated with variability in dye loading,