๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Estimation of hydrogen peroxide in plant extracts using titanium(IV)

โœ Scribed by Brian D. Patterson; Elspeth A. MacRae; Ian B. Ferguson


Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
457 KB
Volume
139
Category
Article
ISSN
0003-2697

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โœฆ Synopsis


Methods for the estimation of hydrogen peroxide in acetone extracts using titanium(IV) are likely to overestimate hydrogen peroxide when applied to plant leaves. Pigments appear to co-precipitate with the titanium complex and cannot be removed by washing with solvents. Fluoride, which specifically removes the color of the titanium-peroxide complex, removes only some of the color from the reactions with plant extracts. This problem has been avoided by extracting tissues with trichloroacetic acid, and measuring peroxide against catalase-treated blanks by its reaction with the complex of titanium(IV) with 4-(2-pyridylazo) resorcinol. Levels of hydrogen peroxide in leaves of a variety of species were found to range from about 0.1 to 0.6 mumol X g-1.


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