Estimation of experimental conditions that permit cell separations by velocity sedimentation on isokinetic gradients of ficoll in tissue culture medium

Separation of megakaryocytes from rat bo

Separation of megakaryocytes from rat bone marrow cells using velocity sedimentation in an isokinetic gradient of ficoll in tissue culture medium

✍ Dr. Thomas G. Pretlow; Anthony J. Stinson 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 422 KB

## Abstract A technique for the purification of rat megakaryocytes is described. Velocity sedimentation in a previously described isokinetic gradient of Ficoll (polysucrose) in tissue culture medium was more effective than isopycnic sedimentation for the purification of megakaryocytes and resulted