Estimation of cholesterol sulphate in blood plasma and in erythrocyte membranes from individuals with Down's syndrome or diabetes mellitus type I
✍ Scribed by M. Przybylska; M. Bryszewska; U. Nowicka; K. Szosland; J. Kȩdziora; R.M. Epand
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 585 KB
- Volume
- 28
- Category
- Article
- ISSN
- 0009-9120
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✦ Synopsis
Objectives: Plasma and erythrocyte membrane cholesterol sulphate (CS) were measured in patients suffering from diabetes and Down's syndrome.
Design and Methods:
The procedure for separation and determination of CS comprised HPTLC (high-performance thin-layer chromatography) and densitometry.
Results:
The mean plasma and RBC membranes CS concentrations (-+SD) of the control group (n = 16) was 188 -+ 47 p,g/dL and 343 -+ 57 p,g/1012 RBC, respectively. In 15 patients with diabetes and 12 Down's syndrome patients substantially higher CS levels were found (diabetes: plasma-348 -+ 60 p,g/dL; RBC membranes-646 --113 FLg/1012 RBC; Down's syndrome: plasma-245 + 54 p.g/dL; RBC membranes 427 -+ 74 p.g/10 ~2 RBC). Analysis of variance and multiple comparison (Newman-Keuls test) show statistically significant differences between all samples both for erythrocytes, F(2,41) = 52.24, p < 0.05, and plasma, F(2,41) = 34.92, p < 0.05.
Conclusions:
It is postulated that differences in CS levels may contribute to changes of erythrocyte properties in these pathological states.