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Estimation of binding parameters for the protein–protein interaction using a site-directed spin labeling and EPR spectroscopy

✍ Scribed by Marcin Sarewicz; Sebastian Szytuła; Małgorzata Dutka; Artur Osyczka; Wojciech Froncisz


Publisher
Springer
Year
2007
Tongue
English
Weight
375 KB
Volume
37
Category
Article
ISSN
1432-1017

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✍ Sonia Longhi; Valérie Belle; André Fournel; Bruno Guigliarelli; Frédéric Carrièr 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 858 KB

## Abstract EPR spectroscopy is a technique that specifically detects unpaired electrons. EPR‐sensitive reporter groups (spin labels or spin probes) can be introduced into biological systems via site‐directed spin‐labeling (SDSL). The basic strategy of SDSL involves the introduction of a paramagnet