Esterase-16 (Es-16): Characterization, polymorphism, and linkage to chromosome 3 of a kidney esterase locus of the house mouse

✍ Scribed by Otto H. Deimling; Peter Schupp; Johannes Otto

• Publisher: Springer
• Year: 1981
• Tongue: English
• Weight: 426 KB
• Volume: 19
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00484567

✦ Synopsis

A polymorphismfor an isozyme of a presumed arylesterase, esterase-16 (EC 3.1.1.2), has been detected in kidney, heart, and spleen of the house mouse, Mus musculus, by means of isoelectric focusing and by disc electrophoresis. Three phenotypes can be distinguished." the ES-16A phenotype (IEP 5.9) was found in C57BL/10Sn and many other laboratory inbred strains; the ES-16B phenotype (IEP 6.1) was found in M. m. molossinus; and the ES-16C phenotype (IEP 5.9; very weak activity) was found in Peru-Coppock. Esterase-16 is strongly inhibited by 10 -3 M p-chloromercuribenzoate, but not by 2 • 10 -4 M bis-p-nitrophenylphosphate or by 10 -3 M Diamox. It stains well with indoxyl acetate and other indigogenic substrates but only weakly with a-naphthyl acetate. Esterase-16 is completely insoluble in water. It is apparently governed by a structural gene locus, Es-16, with three alleles, Es-16 a, Es-16 b, and Es-16 c, respectively. Es-16 is closely linked to Car-1 and Car-2 on chromosome 3. Typing of 94 animals of the backcross (C57BL/ lOSn × M. m. mol.)Fl x M. m. tool. revealed a recombination frequency of 8.51 +_ 2.9%.