Establishment of T-cell Lines from Infiltrated NOD Islets by Stimulation of Specific T-Cell Receptor Vβ Segments
✍ Scribed by Didier Maugendre; Béatrice Legrand; Martine Olivi; Pierre Bedossa; Jean-François Bach; Claude Carnaud
- Book ID
- 115621227
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 480 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0896-8411
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T-cell receptor (TCR) -chain usage in experimental autoimmune encephalomyelitis (EAE) seems to be much more heterogeneous than previously assumed even for a single autoantigen in an inbred animal strain. Owing to the lack of suitable antibodies, this has been demonstrated only at the RNA level so f
## Abstract We have used a new polymerase chain reaction‐based technique to analyze at the clonal level the CDR3 diversity and the Jβ usage associated with the Vβ‐dependent T cell receptor (TCR) recognition of two superantigens: the staphylococcal enterotoxin B and the __Urtica dioica__ agglutinin.