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Establishment of callus, cell suspension and shoot cultures ofLeonurus cardiacaL. and diterpene analysis

✍ Scribed by Werner Knöss

Publisher
Springer
Year
1995
Tongue
English
Weight
310 KB
Volume
14
Category
Article
ISSN
0721-7714

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✦ Synopsis

Callus cultures, cell suspension cultures and shoot cultures of Leonurus cardiaca L. (Motherwort) were established and growth conditions optimized. Shoot cultures showed constant growth whether in the dark or under continuous light, accumulating varying amounts of the furanic labdane diterpenes leosibiricin, preleosibirin, leosibirin and isoballotenol acetate, which are also present in the soil-grown plants. Only traces of leosibiricin were detected in callus cultures, while cell suspension cultures did not produce any furanic diterpenes. A small amount of furanic labdane diterpenes was found in the medium of shoot cultures. Callus and shoot culture induction of several other Lamiaceae species is also described.

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