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Establishment of an ELISA for the Detection of Orthopox Viruses Based on Neutralizing Monoclonal and Polyclonal Antibodies

โœ Scribed by Czerny, C.-P. ;Meyer, H. ;Mahnel, H.


Book ID
115162578
Publisher
John Wiley and Sons
Year
1989
Tongue
English
Weight
602 KB
Volume
36
Category
Article
ISSN
0931-1793

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โœ Barbara Kropff; Maria-Paola Landini; Michael Mach ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 827 KB

Two prokaryotically expressed fusion proteins encompassing amino acids 484-650 (AD-I) and 27-100 (AD-2) of glycoprotein gp58/116 of human cytomegalovirus (HCMV) were purified from E. coli lysates and used in ELISA to determine antibody levels in human sera. The specificity of the test was establishe