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Establishment of an astrocyte progenitor cell line: Induction of glial fibrillary acidic protein and fibronectin by transforming growth factor-β1

✍ Scribed by T. Yoshida; M. Takeuchi

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
839 KB
Volume
35
Category
Article
ISSN
0360-4012

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✦ Synopsis

Abstract

An immortalized clonal cell line (AP‐16) has been established from glial cultures obtained from neonatal mouse cerebra by multipassages under serum‐free conditions. Immunofluorescent experiments showed that AP‐16 cells expressed a marker for glial progenitors (A2B5) and did not express markers for oligodendrocytes (galactocerebroside) or mature astrocytes (glial fibrillary acidic protein: GFAP). Treatment with transforming growth factor‐β1 (TGF‐β1) or fetal calf serum (FCS) for 2 days induced AP‐16 cells to differentiate into A2B5‐negative, GFAP‐positive, phenotypically mature astrocytes. AP‐16 cells depended on epidermal growth factor for survival, and their growth was inhibited by FCS. These results indicate that AP‐16 cells retained the properties of astrocyte progenitors. An enzyme‐linked immunosorbent assay showed that AP‐16 cells synthesized fibronectin and laminin, and that the expression of fibronectin was increased by TGF‐β1. AP‐16 cells should be useful for studying the roles of TGF‐β1 in the differentiation of astrocyte progenitors. © 1993 Wiley‐Liss, Inc.

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