Establishment of a sensitive radioimmunoassay for serum thymic factor with a special reference to extraction procedures

✍ Scribed by Dr. Naofumi Mukaida; Taeko Morimoto; Naoki Takanashi; Tadashi Kawai

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 486 KB
• Volume: 6
• Category: Article
• ISSN: 0887-8013
• DOI: 10.1002/jcla.1860060107

✦ Synopsis

~ ~

A radioimmunoassay (RIA) for serum thymic factor (facteur thymique serique) was established. This assay could detect <0.5 pg of serum thymic factor without the interference by other thymic hormones, including thymopoietin II and thymosin a,. Both zincfree and zinc-bound serum thymic factor showed a similar standard curve, indicating that this RIA could detect both forms of serum thymic factor in the same way. We observed that two procedures were required prior to the measurement in order to measure serum thymic factor content in plasmazhe first, the extraction procedure to remove substances interfering with the assay nonspecifically; the second, the storage of the sample with ethylenediamine tetraacetic acid dissodium salt to inhibit the proteases in the plasma to degrade serum thymic factor. By employing this assay system, we observed that cord blood plasmas contained significantly higher level of serum thymic factor than did adult blood plasmas.