✦ LIBER ✦
Establishment of a rat hepatoma cell line which has ornithine carbamoyltransferase activity and grows continuously in arginine-deprived medium
✍ Scribed by Akira Niwa; Katsuhiko Yamamoto; Yosihiro Yasumura
- Publisher
- John Wiley and Sons
- Year
- 1979
- Tongue
- English
- Weight
- 631 KB
- Volume
- 98
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
The epithelial cell line, H4-11-E derived from Reuber hepatoma H35 has no significant activity of ornithine carbamoyltransferase (OCT, EC 2.1.3.3) and is not able to grow in arginine-deprived medium.
A multi-step selection procedure is described which selects from H4-11-E populations, cells with OCT activity which thine-supplemented media.