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Establishment of a new method, transcription–reverse transcription concerted reaction, for detection of plasmahnRNP B1mRNA, a biomarker of lung cancer

✍ Scribed by Akemi Sato; Naoko Sueoka-Aragane; Juichi Saitoh; Kazutoshi Komiya; Takashi Hisatomi; Rika Tomimasu; Shinichiro Hayashi; Eisaburo Sueoka

Publisher
Springer-Verlag
Year
2008
Tongue
English
Weight
453 KB
Volume
134
Category
Article
ISSN
1432-1335

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## Abstract There are several methods available to analyze the mRNA concentration quantitatively. Among them, the competitive reverse transcription (RT‐)PCR method is very useful. For this method, Cy5‐labeled primers were used, and after gel electrophoresis in 7 M urea, the Cy5‐labeled single‐stran