Establishment of a human cell line (HCC-T) from a patient with hepatoma bearing no evidence of hepatitis B or A virus infection

✍ Scribed by Hidetsugu Saito; Toshio Morizane; Tetsu Watanabe; Tatehiro Kagawa; Naoto Iwabuchi; Naoki Kumagai; Yasutaka Inagaki; Kanji Tsuchimoto; Masaharu Tsuchiya

• Publisher: John Wiley and Sons
• Year: 1989
• Tongue: English
• Weight: 817 KB
• Volume: 64
• Category: Article
• ISSN: 0008-543X
• DOI: 10.1002/1097-0142(19890901)64:5<1054::aid-cncr2820640516>3.0.co;2-x

✦ Synopsis

A human hepatoma cell line, designated HCC-T, was established. The tumor was surgically obtained from a Japanese male patient with hepatocellular carcinoma (HCC) arising in a cirrhotic liver that had supposedly developed from nonAnonB (NANB) chronic hepatitis. HCC-T exhibited a typical morphology of epithelial cells in culture. Population doubling time was 24 hours and HCC-T cells had characteristics of malignant cells demonstrated by the ability to grow in a soft agar medium and transplantability to nude mice. The histologic condition of the tumor transplanted to a nude mouse showed similarity to the original tumor. A chromosome analysis showed that there were ten identifiable marker chromosomes and sex chromosomes with its modal number of 64. Alpha-fetoprotein (AFP) production was demonstrated by direct immunofluorescence study, but albumin or hepatitis B surface antigen were not detectable. The integration of hepatitis B viral DNA was not demonstrable in the genome of HCC-T cells or the original hepatoma.

Cancer 64:1054-1060, 1989.

HE INCIDENCE of hepatocellular carcinoma (HCC)

T has steadily increased in Japan. However, it is not only a local problem but also a global problem, especially in Africa and Asia where its prevalence is high.' Epidemiologic studies have suggested that there is a close association between persistent hepatitis B virus (HBV) infection and the development of HCC2x3 Recent advances in molecular biology suggest that the integration of HBV DNA to liver cell DNA might be related to malignant transformation of liver cells to HCC cell^.^-^ The contribution of human HCC cell lines, such as PLC/PRF/S or HCC-M that were derived from patients carrying HBV, to these studies4r6 should be appropriately estimated.

It is well recognized that HCC also develops in cases of liver cirrhosis or chronic hepatitis in which there is no From the