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Establishment of a continuous tumor-cell line (PANC-1) from a human carcinoma of the exocrine pancreas

✍ Scribed by Michael Lieber; Joann Mazzetta; Walter Nelson-Rees; Michael Kaplan; George Todaro

Publisher
John Wiley and Sons
Year
1975
Tongue
French
Weight
708 KB
Volume
15
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

An epithelioid cell line, started from a human pancreatic carcinoma of ductal cell origin, has been maintained in culture for over 2 years and has been subcultured more than 40 times. The PANC‐1 cell line has a doubling time of 52 h and G6PD activity of the slow mobility or B type. Chromosome studies show a modal number of 63 with three distinct marker chromosomes and a small ring chromosome. The malignant nature of the PANC‐1 cell line was verified by: (1) the ready growth of PANC‐1 cells in soft agar and on top of a fibroblast monolayer; and (2) the formation of a progressively growing anaplastic carcinoma after injection of a nude‐athymic mouse with PANC‐1 cells.

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