Establishment in culture of a multihormone-secreting cell strain derived from the mtT/F4 rat pituitary tumor
✍ Scribed by U. Ingrid Richardson
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- English
- Weight
- 769 KB
- Volume
- 88
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
A clonal cell strain F4C1 has been established from the transplantable rat pituitary tumor MtT/F4 and has been maintained i n continuous culture for two years. The cells grow with a population doubling time of 48 hours; the karyotype with a modal number of 39 chromosomes includes a pair of large metacentric marker chromosomes. F4C1 cells in culture produce growth hormone and prolactin but not adrenocorticotropin in contrast to the MtT/F4 tumor which secretes all three hormones in the host rat. The cloned cells lack specific receptors for thyrotropin-releasing hormone and do not respond to this agent with increased prolactin or decreased growth hormone production. Treatment with hydrocortisone results i n a small increase in growth hormone and a small decrease i n prolactin production. Tumors generated i n rats from injected F4C1 cells secrete prolactin and growth hormone but not adrenocorticotropin. The results suggest that growth hormone and prolactin are produced by a single cell type in the MtT/F4 tumor.