Establishment, characterization and drug sensitivity of four new human soft tissue sarcoma cell lines

✍ Scribed by Wei-Wei Li; Carlos Cordon-Cardo; Quanguang Chen; Suresh C. Jhanwar; Joseph R. Bertino

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: French
• Weight: 727 KB
• Volume: 68
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19961115)68:4<514::aid-ijc18>3.0.co;2-4

✦ Synopsis

Four new cell lines were established from patients with soft tissue sarcomas. Drug sensitivity as well as genotypic characterization, which may be related to drug sensitiviky in these cell lines, was determined. Karyotype, H-ras, c-myc and mutant p53 gene expression, Rb, GI-and S-phase cyclins, E2F and major cyclin/CDK inhibitors such as p16 and p21 and p-glycoprotein were analyzed using cytogenetic, Northern blot and immunological methods. Drug sensitivity was determined using growth inhibition tests. These cell lines differed in their morphology and growth rates, forming colonies in soft agar with a cloning efficiency of 4.3-13.4%. and 3 of the 4 cell lines grew in nude mice. Cytogenetic analysis of cell lines revealed highly aneuploid karyotypes. Deletion and/or translocation of chromosome 17 was seen in HS-16, HS-18 and HS-30 cells, and both copies of chromosome I 3 were lost or marranged in the HS-I8 cell line. Mutant p53 protein was present in all 4 cell lines. HS-I8 cells showed no expression of the Rb protein and high levels of expression of EZF, cyclin A, cyclin E and CDKZ. HS-I6 expressed a higher level of cyclin D than the other 3 cell lines. p2Id1 expression was seen in all cell lines, but p16'*' was expressed only in HS-30 and HS-42 cell lines. These cell lines were sensitive to tax01 and relatively resistant to methotrexate, vinblastine and 5-fluorouracil when compared with the fib-coma cell line HT-1080. These new cell lines should provide a useful model for the study of soft tissue sarcomas and for evaluating new drugs or treatments.