✦ LIBER ✦
Establishment and growth characteristics of a cell suspension culture ofMarchantia polymorphaL. with high chlorophyll content
✍ Scribed by Yoshimoto Ohta; Kenji Katoh; Keiko Miyake
- Book ID
- 104749731
- Publisher
- Springer-Verlag
- Year
- 1977
- Tongue
- English
- Weight
- 766 KB
- Volume
- 136
- Category
- Article
- ISSN
- 0032-0935
No coin nor oath required. For personal study only.
✦ Synopsis
A green-pigmented cell suspension culture of Marchantia polymorpha was established using the medium of Murashige and Skoog with addition of organic acids of the tricarboxylic-acid cycle, vitamins and sugars plus sugar alcohols, exclusion of kinetin, and replacement of sucrose with glucose. In continuous light, the cells grew exponentially for ca. 10 days; in the dark, they grew only to a slight extent. The light-grown cells contained well-developed chloroplasts, and chlorophyll content reached almost twice that of the intact gametophyte.