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Establishment and characterization of long-term primary mouse urothelial cell cultures

✍ Scribed by van der Kwast, T. H. ;van Rooy, H. ;Mulder, A. H.

Publisher
Springer
Year
1989
Tongue
English
Weight
470 KB
Volume
17
Category
Article
ISSN
0300-5623

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✦ Synopsis

Long-term mouse urothelial cell cultures were routinely established from explants of neonatal mouse bladders. Foci of proliferating cells could be observed one week after the initiation of the explant cultures. These persisted throughout the culture period and up to one year. Expression of keratin proteins confirmed the epithelial nature of the cultured cells. Morphologic analysis of nuclei sorted after DNA flow cytometry revealed a population of DNA-tetraploid and octoploid cells with large nuclei and prominent nucleoli in addition to a DNA-diploid cell population. Both cell populations showed DNA replicative activity as reflected by bromodeoxyuridine incorporation studies and mitotic activity. These long-term primary mouse urothelial cell cultures may prove useful for studies on urothelial cell kinetics and bladder carcinogenesis.

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✍ Rahman, Z. ;Reedy, A. ;Heatfield, B.M. πŸ“‚ Article πŸ“… 1987 πŸ› Springer 🌐 English βš– 597 KB

Trypsinization (WT) was employed to disaggregate urothelial cells from normal human urinary bladder mucosa for the preparation of primary cultures. Urinary bladders of two male adults both 25 years old were obtained autopsy 1-2 h after death. The mucosa was incubated in HBSS containing 0.25% trypsin