Establishment and characterization of a new human B-cell line (ONHL-1) from non-Hodgkin's lymphoma: Constant expression of bcl-2 gene during mitogen-induced growth inhibition
✍ Scribed by Itaru Matsumura; Toshiharu Tamaki; Shuichi Katagiri; Masafumi Taniwaki; Nobuhiko Tominaga; Kenji Oritani; Masato Iida; Hirosuke Yagura; Takeshi Yonezawa; Seiichiro Tarui
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- French
- Weight
- 973 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
A new 6-cell line (ONHL-I) was established from non-Hodgkin's lymphoma. ONHL-I was free from Epstein-Barr virus nuclear antigen and expressed CDZO, CD24, and slg (p, 8, y and K), thus being equivalent to the mature 6-cell stage.
Chromosome analysis revealed a markedly abnormal pattern including 14q+ and 6q-. In accordance with the positive expression of surface K light chains, one of the K genes was found to be rearranged. However, rearrangement of the A locus was also detected, contrary to the supposed hierarchy for the rearrangement of the light-chain genes. The cell line showed rearrangement of the bcl-2 gerre. Further, the rearranged fragments of the JH! C,, and bcl-;! genes were of the same size in the EcoRl and Hindlll digests on the same filter. This may suggest that the bcl-2 gene is juxtaposed with the JH and C, locus. The proliferation of ONHIL-I was inhibited by adding Staphylococcus aureus Cowan I or 12-Otetradecanoyl-phorbol-13-acetate. During this growth inhibition, the expression of c-myc decreased, while that of bcl-2 mRNA remained steady. This result suggests that not the bcl-2 gene but other oncogenes, such as c-myc, play a key role in the proliferation of ONHL-I. This agrees with the hypothesis that the bcl-2 gene is not concerned with aggressive proliferation but with cell survival. This new cell line 'will therefore be of value in studying the differentiation and tumorigenesis of 6 cells.