Establishing styrylpyrone synthase activity in cell free extracts obtained from gametophytes of Equisetum arvense L. by high performance liquid chromatography–tandem mass spectrometry
✍ Scribed by Markus Herderich; Cornelia Beckert; Markus Veit
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 73 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0958-0344
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✦ Synopsis
Styrylpyrone synthase is a polyketide synthase, catalyzing the formation of styrylpyrones from hydroxycinnamoyl-CoA precursors and malonyl-CoA. After incubation of cell free extracts obtained from Equisetum arvense L. gametophytes with p-coumaroyl-CoA or caffeoyl-CoA, high performance liquid chromatography atmospheric pressure chemical ionization-tandem mass spectrometry (HPLC-APCI-MS) led to the identification of bisnoryangonin and hispidin, respectively, as metabolites. These findings enabled the development of an assay for styrylpyrone synthase activity based on HPLC analysis of the reaction products. The technique could also be useful for product identification in related polyketide synthase reactions.